Article
Comparison of Sanger sequencing, pyrosequencing, and melting curve analysis for the detection of KRAS mutations: diagnostic and clinical implications.
The Journal of molecular diagnostics : JMD - 1 Jul 2010
Tsiatis Athanasios C, Norris-Kirby Alexis, Rich Roy G, Hafez Michael J, Gocke Christopher D, Eshleman James R, Murphy Kathleen M
Abstract excerpt
Mutations in codons 12 and 13 of the KRAS oncogene are relatively common in colorectal and lung adenocarcinomas. Recent data indicate that these mutations result in resistance to anti-epidermal growth factor receptor therapy. Therefore, we assessed Sanger sequencing, pyrosequencing, and melting curve analysis for the detection of KRAS codon 12/13 mutations in formalin-fixed paraffin-embedded samples, including 58...
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