Article
Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency.
Genome biology - 15 Dec 2015
Dang Ying, Jia Gengxiang, Choi Jennie, Ma Hongming, Anaya Edgar, Ye Chunting, Shankar Premlata, Wu Haoquan
Abstract excerpt
BACKGROUND: Single-guide RNA (sgRNA) is one of the two key components of the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome-editing system. The current commonly used sgRNA structure has a shortened duplex compared with the native bacterial CRISPR RNA (crRNA)-transactivating crRNA (tracrRNA) duplex and contains a continuous sequence of thymines, which is the pause signal for RNA...
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