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Article

Enhancer scanning to locate regulatory regions in genomic loci.

Nature protocols - 1 Jan 2016

Buckley Melissa, Gjyshi Anxhela, Mendoza-Fandiño Gustavo, Baskin Rebekah, Carvalho Renato S, Carvalho Marcelo A, Woods Nicholas T, Monteiro Alvaro N A

Abstract excerpt

This protocol provides a rapid, streamlined and scalable strategy to systematically scan genomic regions for the presence of transcriptional regulatory regions that are active in a specific cell type. It creates genomic tiles spanning a region of interest that are subsequently cloned by recombination into a luciferase reporter vector containing the simian virus 40 promoter. Tiling clones are transfected into...

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Identifiers

PMID: 26658467
DOI: 10.1038/nprot.2015.136
PMCID: PMC4756653

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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