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Article

An ultra-dense library resource for rapid deconvolution of mutations that cause phenotypes in Escherichia coli.

Nucleic acids research - 18 Mar 2016

Nehring Ralf B, Gu Franklin, Lin Hsin-Yu, Gibson Janet L, Blythe Martin J, Wilson Ray, Bravo Núñez María Angélica, Hastings P J, Louis Edward J, Frisch Ryan L, Hu James C, Rosenberg Susan M

Abstract excerpt

With the wide availability of whole-genome sequencing (WGS), genetic mapping has become the rate-limiting step, inhibiting unbiased forward genetics in even the most tractable model organisms. We introduce a rapid deconvolution resource and method for untagged causative mutations after mutagenesis, screens, and WGS in Escherichia coli. We created Deconvoluter-ordered libraries with selectable insertions every 50...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26578563
DOI: 10.1093/nar/gkv1131
PMCID: PMC4797258

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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