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Article

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.

Molecular systems biology - 1 Jan 2006

Baba Tomoya, Ara Takeshi, Hasegawa Miki, Takai Yuki, Okumura Yoshiko, Baba Miki, Datsenko Kirill A, Tomita Masaru, Wanner Barry L, Mori Hirotada

Abstract excerpt

We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes...

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PMID: 16738554
DOI: 10.1038/msb4100050
PMCID: PMC1681482

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