Article
Identification of mutations in laboratory-evolved microbes from next-generation sequencing data using breseq.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Deatherage Daniel E, Barrick Jeffrey E
Abstract excerpt
Next-generation DNA sequencing (NGS) can be used to reconstruct eco-evolutionary population dynamics and to identify the genetic basis of adaptation in laboratory evolution experiments. Here, we describe how to run the open-source breseq computational pipeline to identify and annotate genetic differences found in whole-genome and whole-population NGS data from haploid microbes where a high-quality reference...
Topics
- Directed Molecular Evolution
- Genomics
- High-Throughput Nucleotide Sequencing
- Microbiological Techniques
- Mutation
- Sequence Analysis, DNA
- Software