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Article

Potential confounding mutations in Keio knockout strains: implications for research accuracy.

Microbiology spectrum - 6 May 2025

Sen Oishi, Liu Xianghui, Kjelleberg Staffan, Rice Scott A, Seviour Thomas

Abstract excerpt

The Keio library of single-gene knock-out mutants of Escherichia coli is useful for the research community. It has been used to analyze the role of various E. coli genes in alcohol tolerance, multi-drug resistance, and biofilm formation. The current study provides a general overview of potential single nucleotide polymorphisms (SNPs), insertion-deletion of bases (≤50 nucleotides, INDELs) in the genome of a set of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 40162750
DOI: 10.1128/spectrum.02036-24
PMCID: PMC12054083

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