Article
Rapid whole-genome mutational profiling using next-generation sequencing technologies.
Genome research - 1 Oct 2008
Smith Douglas R, Quinlan Aaron R, Peckham Heather E, Makowsky Kathryn, Tao Wei, Woolf Betty, Shen Lei, Donahue William F, Tusneem Nadeem, Stromberg Michael P, Stewart Donald A, Zhang Lu, Ranade Swati S, Warner Jason B, Lee Clarence C, Coleman Brittney E, Zhang Zheng, McLaughlin Stephen F, Malek Joel A, Sorenson Jon M, Blanchard Alan P, Chapman Jarrod, Hillman David, Chen Feng, Rokhsar Daniel S, McKernan Kevin J, Jeffries Thomas W, Marth Gabor T, Richardson Paul M
Abstract excerpt
Forward genetic mutational studies, adaptive evolution, and phenotypic screening are powerful tools for creating new variant organisms with desirable traits. However, mutations generated in the process cannot be easily identified with traditional genetic tools. We show that new high-throughput, massively parallel sequencing technologies can completely and accurately characterize a mutant genome relative to a...
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