Article
Unlocking the bottleneck in forward genetics using whole-genome sequencing and identity by descent to isolate causative mutations.
PLoS genetics - 1 Jan 2013
Bull Katherine R, Rimmer Andrew J, Siggs Owen M, Miosge Lisa A, Roots Carla M, Enders Anselm, Bertram Edward M, Crockford Tanya L, Whittle Belinda, Potter Paul K, Simon Michelle M, Mallon Ann-Marie, Brown Steve D M, Beutler Bruce, Goodnow Christopher C, Lunter Gerton, Cornall Richard J
Abstract excerpt
Forward genetics screens with N-ethyl-N-nitrosourea (ENU) provide a powerful way to illuminate gene function and generate mouse models of human disease; however, the identification of causative mutations remains a limiting step. Current strategies depend on conventional mapping, so the propagation of affected mice requires non-lethal screens; accurate tracking of phenotypes through pedigrees is complex and...
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