Active site diversification of a non-canonical amino acid decarboxylase by merging substrate multiplexed screening with computationally guided recombination.

Abstract excerpt

Recombination of active site mutations is a powerful strategy to alter enzyme activity. The vastness of sequence space, however, often limits screening-based engineering to single and double site libraries. Here, we explore focused recombination across five positions that enclose the active site of a tryptophan (Trp) decarboxylase. Our goal was to maximize the sequence diversity of enzymes that decarboxylate...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Catalytic Domain
• Share to Models, Molecular