Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

DNA shuffling of uricase gene leads to a more "human like" chimeric uricase with increased uricolytic activity.

International journal of biological macromolecules - 1 Jan 2016

Chen Jing, Jiang Nan, Wang Tao, Xie Guangrong, Zhang Zhilai, Li Hui, Yuan Jing, Sun Zengxian, Chen Jianhua

Abstract excerpt

Urate oxidase (Uox) is the enzyme involved in purine metabolism. Pseudogenization of Uox gene is the underlying mechanism of hyperuricemia and gout in human. Although Uox from various microorganisms has been used in clinical practice for many years, its application is limited by potential immunogenicity. In order to develop a more "human like" uricase, DNA shuffling was used to create chimeric uricase with both...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Substitution
Share to DNA Shuffling

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Enzyme Activation

Share to Gene Library

Share to Hydrolysis

Share to Protein Conformation

Share to Sequence Alignment

Share to Uric Acid

Source

Identifiers

PMID: 26526169
DOI: 10.1016/j.ijbiomac.2015.10.053

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

DNA shuffling of uricase gene leads to a more "human like" chimeric uricase with increased uricolytic activity.PMID 26526169Read ↗

Rationalization design, soluble expression and PEG modification of highly active recombinant human-porcine uricase mutant protein.Semantic proximity Read ↗

Soluble expression of bioactive recombinant porcine-human chimeric uricase mutant employing MBP-SUMO fusion system.Semantic proximity Read ↗

Rational design of a genome‐based insulated system in Escherichia coli facilitates heterologous uricase expression for hyperuricemia treatmentSemantic proximity Read ↗

Structure-based characterization of canine-human chimeric uricases and its evolutionary implications.Semantic proximity Read ↗

Engineering Human Urate Oxidase: Towards Reactivating It as an Important Therapeutic Enzyme.Semantic proximity Read ↗

Catalysis and Structure of Zebrafish Urate Oxidase Provide Insights into the Origin of Hyperuricemia in HominoidsSemantic proximity Read ↗

Heterologous expression and enzymatic properties of the urate oxidase gene from Aspergillus cristatus.Semantic proximity Read ↗

The role of Gln269Leu mutation on the thermostability and structure of uricase from Aspergillus flavusSemantic proximity Read ↗

[Application of a model for gene local shuffling in ubiA mutation].Semantic proximity Read ↗

Discovery of human-like L-asparaginases with potential clinical use by directed evolutionSemantic proximity Read ↗

Alteration of the quaternary structure of human UDP-glucose dehydrogenase by a double mutation.Semantic proximity Read ↗

Uric acid in plants and microorganisms: Biological applications and genetics - A reviewSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.