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The role of Gln269Leu mutation on the thermostability and structure of uricase from Aspergillus flavus

10 Mar 2025

Abstract excerpt

Aspergillus flavus Urate oxidase (AFUOX) is promising for potential therapeutic applications, particularly in gout treatment. However, the enzyme’s low thermostability and solubility limit its efficacy. A targeted mutation, substituting Gln with Leu at position 269 (Q269L) has been proposed to enhance its stability. The turnover number, catalytic efficiency, and specific activity of Q269L were 3.7 (s-1), 53.2...

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Share to Gout, Hyperuricemia, Uric Acid
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PMID: 40064946
DOI: 10.1038/s41598-025-89605-w

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