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Article

Soluble expression of bioactive recombinant porcine-human chimeric uricase mutant employing MBP-SUMO fusion system.

Protein expression and purification - 1 Jan 2022

Zhou Zhenlong, Zhao Hui, Zhang Ligang, Xie Qiuling, Liu Qiwei, Tong Mingjie, Yu Xiangwei, Xiong Sheng

Abstract excerpt

Urate oxidase is a promising biological medicine for hyperuricemia treatment, but immunogenicity obstructs the development of its clinical application. The recombinant porcine-human chimeric uricase mutant named dHU-wPU is a humanized chimeric uricase based on wild porcine uricase (wPU), which can effectively reduce the limitation of potential immunogenicity with a high homology (92.76%) to deduced human uricase...

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PMID: 34562586
DOI: 10.1016/j.pep.2021.105978

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