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Article

Heterologous expression and enzymatic properties of the urate oxidase gene from Aspergillus cristatus.

Enzyme and microbial technology - 1 Sept 2026

Liu Kaihui, Liu Yanhong, Ding Xiaowei

Abstract excerpt

This study reports the first molecular cloning of the urate oxidase (UOX) gene from Aspergillus cristatus, yielding a novel oxidase with potential clinical applications. Following codon optimization, the wild-type UOX gene was synthesized and cloned into the pET-28a(+) vector for expression in Escherichia coli BL21(DE3). Among the five mutants generated using a multi-step prioritization strategy, the Y243K mutant...

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PMID: 42275861
DOI: 10.1016/j.enzmictec.2026.110922

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