Article
SMRT Sequencing for Parallel Analysis of Multiple Targets and Accurate SNP Phasing.
G3 (Bethesda, Md.) - 23 Oct 2015
Guo Xiaoge, Lehner Kevin, O'Connell Karen, Zhang Jenny, Dave Sandeep S, Jinks-Robertson Sue
Abstract excerpt
Single-molecule real-time (SMRT) sequencing generates much longer reads than other widely used next-generation (next-gen) sequencing methods, but its application to whole genome/exome analysis has been limited. Here, we describe the use of SMRT sequencing coupled with barcoding to simultaneously analyze one or a small number of genomic targets derived from multiple sources. In the budding yeast system, SMRT...
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