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Highly sensitive detection of mutations in CHO cell recombinant DNA using multi-parallel single molecule real-time DNA sequencing.

Biotechnology and bioengineering - 1 Jun 2018

Cartwright Joseph F, Anderson Karin, Longworth Joseph, Lobb Philip, James David C

Abstract excerpt

High-fidelity replication of biologic-encoding recombinant DNA sequences by engineered mammalian cell cultures is an essential pre-requisite for the development of stable cell lines for the production of biotherapeutics. However, immortalized mammalian cells characteristically exhibit an increased point mutation frequency compared to mammalian cells in vivo, both across their genomes and at specific loci...

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PMID: 29427433
DOI: 10.1002/bit.26561

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