Article
Genome editing by natural genetic transformation in Streptococcus mutans.
Journal of microbiological methods - 1 Dec 2015
Morrison D A, Khan R, Junges R, Åmdal H A, Petersen F C
Abstract excerpt
Classical mutagenesis strategies using selective markers linked to designed mutations are powerful and widely applicable tools for targeted mutagenesis via natural genetic transformation in bacteria and archaea. However, the markers that confer power are also potentially problematic as they can be cumbersome, risk phenotypic effects of the inserted genes, and accumulate as unwanted genes during successive...
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