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Article

Genetic Manipulation of Group A Streptococcus-Gene Deletion by Allelic Replacement.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Barnett Timothy C, Daw Jessica N, Walker Mark J, Brouwer Stephan

Abstract excerpt

Genetic manipulation of Streptococcus pyogenes (Group A Streptococcus, GAS) has historically been a challenging process, with considerable variation in efficiency between different strains. Here, we outline an optimized, rapid method for creating markerless isogenic mutations that combines Gibson assembly cloning with a new temperature-sensitive plasmid, pLZts. This method is highly efficient and reduces the time...

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PMID: 32430813
DOI: 10.1007/978-1-0716-0467-0_5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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