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Article

Recombineering in Streptococcus mutans Using Direct Repeat-Mediated Cloning-Independent Markerless Mutagenesis (DR-CIMM).

Frontiers in cellular and infection microbiology - 1 Jan 2017

Zhang Shan, Zou Zhengzhong, Kreth Jens, Merritt Justin

Abstract excerpt

Studies of the dental caries pathogen Streptococcus mutans have benefitted tremendously from its sophisticated genetic system. As part of our own efforts to further improve upon the S. mutans genetic toolbox, we previously reported the development of the first cloning-independent markerless mutagenesis (CIMM) system for S. mutans and illustrated how this approach could be adapted for use in many other organisms....

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 28589101
DOI: 10.3389/fcimb.2017.00202
PMCID: PMC5440470

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