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Streamlining marker-less allelic replacement in Streptococcus pneumoniae through a single transformation step strategy: easyJanus.

Applied and environmental microbiology - 18 Sept 2024

Chembilikandy Vipin, D'Mello Adonis, Tettelin Hervé, Martínez Eriel, Orihuela Carlos J

Abstract excerpt

The ability to genetically manipulate bacteria is a staple of modern molecular microbiology. Since the 2000s, marker-less mutants of Streptococcus pneumoniae (Spn) have been made by allelic exchange predominantly using the kanR-rpsL cassette known as "Janus." The conventional Janus protocol involves two transformation steps using multiple PCR-assembled products containing the Janus cassette and the target gene's...

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Identifiers

PMID: 39140740
DOI: 10.1128/aem.01010-24
PMCID: PMC11409681

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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