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Article

Markerless Genome Editing in Competent Streptococci.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Junges Roger, Khan Rabia, Tovpeko Yanina, Åmdal Heidi A, Petersen Fernanda C, Morrison Donald A

Abstract excerpt

Selective markers employed in classical mutagenesis methods using natural genetic transformation can affect gene expression, risk phenotypic effects, and accumulate as unwanted genes during successive mutagenesis cycles. In this chapter, we present a protocol for markerless genome editing in Streptococcus mutans and Streptococcus pneumoniae achieved with an efficient method for natural transformation. High yields...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 27924598
DOI: 10.1007/978-1-4939-6685-1_14

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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