Article
Design of a New Fluorescent Oligonucleotide-Based Assay for a Highly Specific Real-Time Detection of Apurinic/Apyrimidinic Site Cleavage by Tyrosyl-DNA Phosphodiesterase 1.
Bioconjugate chemistry - 21 Oct 2015
Lebedeva Natalia A, Anarbaev Rashid O, Kupryushkin Maxim S, Rechkunova Nadejda I, Pyshnyi Dmitrii V, Stetsenko Dmitry A, Lavrik Olga I
Abstract excerpt
Tyrosyl-DNA phosphodiesterase 1 (Tdp1) promotes catalytic scission of a phosphodiester bond between the 3'-end of DNA and the hydroxyl group of a tyrosine residue, as well as cleaving off a variety of other 3'-terminal phosphate-linked DNA substituents. We have shown recently that Tdp1 can initiate an apurinic/apyrimidinic (AP) site repair pathway that is independent from the one mediated by AP endonuclease 1...
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