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Article

Detection of single-base mutations by fluorogenic ribonuclease protection assay.

Analytical chemistry - 1 Nov 2005

Ichinose Hirofumi, Kitaoka Momoko, Okamura Nobuko, Maruyama Tatsuo, Kamiya Noriho, Goto Masahiro

Abstract excerpt

The ribonuclease protection assay is a generally applicable technique for the detection of known mutations. We have developed a simple and rapid method for mutation detection based on the ribonuclease protection assay using fluorescently labeled oligodeoxyribonucleotide probes. The fluorogenic ribonuclease protection (FRAP) assay uses two differently labeled oligodeoxyribonucleotides, a donor probe and an...

Topics

Base Pair Mismatch
Base Sequence
Fluorescence Resonance Energy Transfer
Hepatitis B virus
Polymorphism, Single Nucleotide
Ribonucleases
Sensitivity and Specificity

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 16255608
DOI: 10.1021/ac050782k

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