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Article

Development of an oligonucleotide-based fluorescence assay for the identification of tyrosyl-DNA phosphodiesterase 1 (TDP1) inhibitors.

Analytical biochemistry - 1 Jun 2014

Walker Sarah, Meisenberg Cornelia, Bibby Rachel A, Askwith Trevor, Williams Gareth, Rininsland Frauke H, Pearl Laurence H, Oliver Antony W, El-Khamisy Sherif, Ward Simon, Atack John R

Abstract excerpt

Topoisomerase 1 (TOP1) generates transient nicks in the DNA to relieve torsional stress encountered during the cellular processes of transcription, replication, and recombination. At the site of the nick there is a covalent linkage of TOP1 with DNA via a tyrosine residue. This reversible TOP1-cleavage complex intermediate can become trapped on DNA by TOP1 poisons such as camptothecin, or by collision with...

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Identifiers

PMID: 24637157
DOI: 10.1016/j.ab.2014.03.004
PMCID: PMC4007590

Genes

TDP1
TOP1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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