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Article

Solid-Phase Hybridization Assay for Detection of Mutated Cancer DNA by Fluorescence.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Taskova Maria, Astakhova Kira

Abstract excerpt

We report a straightforward protocol for the detection of mutated DNA extracted from cancer cells. The assay combines a step-wise solid-phase hybridization and a readout by fluorescence emission. We detect a single-nucleotide polymorphism in two human oncogenes, BRAF and EGFR, and reach a limit of the detection of 300 pM by conventional fluorometry. The protocol described herein may be used as a foundation for...

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PMID: 31667761
DOI: 10.1007/978-1-0716-0138-9_4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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