Article
Automated N-glycan profiling of a mutant Trypanosoma rangeli sialidase expressed in Pichia pastoris, using tandem mass spectrometry and bioinformatics.
Glycobiology - 1 Dec 2015
Li Haiying, Rasmussen Morten I, Larsen Martin R, Guo Yao, Jers Carsten, Palmisano Giuseppe, Mikkelsen Jørn D, Kirpekar Finn
Abstract excerpt
A mutant Trypanosoma rangeli sialidase, Tr7, expressed in Pichia pastoris, exhibits significant trans-sialidase activity, and has been used for analytical-scale production of sialylated human milk oligosaccharides. Mass spectrometry-based site-specific N-glycoprofiling of Tr7 showed that heterogeneous high-mannose type N-glycans were present at all the five potential N-linked glycosites. N-linked glycans in Tr7...
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