Article
Design of Trypanosoma rangeli sialidase mutants with improved trans-sialidase activity.
PloS one - 1 Jan 2017
Nyffenegger Christian, Nordvang Rune Thorbjørn, Jers Carsten, Meyer Anne S, Mikkelsen Jørn Dalgaard
Abstract excerpt
A sialidase (EC 3.2.1.18) from the non-pathogenic Trypanosoma rangeli, TrSA, has been shown to exert trans-sialidase activity after mutation of five specific amino acids in the active site (M96V, A98P, S120Y, G249Y, Q284P) to form the so-called TrSA5mut enzyme. By computational and hypothesis driven approaches additional mutations enhancing the trans-sialidase activity have been suggested. In the present work, we...
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