Article
Development of Transcriptomic Markers for Population Analysis Using Restriction Site Associated RNA Sequencing (RARseq).
PloS one - 1 Jan 2015
Alabady Magdy S, Rogers Willie L, Malmberg Russell L
Abstract excerpt
We describe restriction site associated RNA sequencing (RARseq), an RNAseq-based genotype by sequencing (GBS) method. It includes the construction of RNAseq libraries from double stranded cDNA digested with selected restriction enzymes. To test this, we constructed six single- and six-dual-digested RARseq libraries from six F2 pitcher plant individuals and sequenced them on a half of a Miseq run. On average, the...
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