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Innovations in double digest restriction-site associated DNA sequencing (ddRAD-Seq) method for more efficient SNP identification.

Analytical biochemistry - 1 Feb 2023

Magbanua Zenaida V, Hsu Chuan-Yu, Pechanova Olga, Arick Mark, Grover Corrinne E, Peterson Daniel G

Abstract excerpt

We present an improved ddRAD-Seq protocol for identifying single nucleotide polymorphisms (SNPs). It utilizes selected restriction enzyme digestion fragments, quick acting ligases that are neutral with the restriction enzyme buffer eliminating buffer exchange steps, and adapters designed to be compatible with Illumina index primers. Library amplification and barcoding are completed in one PCR step, and magnetic...

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PMID: 36481242
DOI: 10.1016/j.ab.2022.115001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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