Article
Genotyping for Species Identification and Diversity Assessment Using Double-Digest Restriction Site-Associated DNA Sequencing (ddRAD-Seq).
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020
Severn-Ellis Anita A, Scheben Armin, Neik Ting Xiang, Saad Nur Shuhadah Mohd, Pradhan Aneeta, Batley Jacqueline
Abstract excerpt
Genotyping-by-sequencing (GBS) is a powerful approach for studying the genetic diversity of legume species. By using restriction enzymes or other methods to generate a reduced representation of the genome for sequencing, GBS can provide genome-wide single nucleotide polymorphisms (SNP) for diversity analysis at high throughput and low cost. Here we describe a novel double-digest restriction site-associated DNA...
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