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Article

Degenerate oligonucleotide primer MIG-seq: an effective PCR-based method for high-throughput genotyping.

The Plant journal : for cell and molecular biology - 1 Jun 2024

Nishimura Kazusa, Kokaji Hiroyuki, Motoki Ko, Yamazaki Akira, Nagasaka Kyoka, Mori Takashi, Takisawa Rihito, Yasui Yasuo, Kawai Takashi, Ushijima Koichiro, Yamasaki Masanori, Saito Hiroki, Nakano Ryohei, Nakazaki Tetsuya

Abstract excerpt

Next-generation sequencing (NGS) library construction often involves using restriction enzymes to decrease genome complexity, enabling versatile polymorphism detection in plants. However, plant leaves frequently contain impurities, such as polyphenols, necessitating DNA purification before enzymatic reactions. To overcome this problem, we developed a PCR-based method for expeditious NGS library preparation,...

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PMID: 38459738
DOI: 10.1111/tpj.16708

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