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Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Aguirre Natalia Cristina, Filippi Carla Valeria, Vera Pablo Alfredo, Puebla Andrea Fabiana, Zaina Giusi, Lia Verónica Viviana, Marcucci Poltri Susana Noemí, Paniego Norma Beatriz

Abstract excerpt

Double digest restriction-site associated DNA sequencing (ddRADseq) technology combines genome reduced representation by digestion with two restriction enzymes and next generation sequencing (NGS) to obtain thousands of markers (SNP, SSR, and InDels) and genotype tens to hundreds of samples simultaneously. In this chapter, we describe a 96-plex derived ddRADseq protocol that can be set up to obtain different...

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PMID: 36781634
DOI: 10.1007/978-1-0716-3024-2_4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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