Article
Kinetic analysis of bypass of abasic site by the catalytic core of yeast DNA polymerase eta.
Mutation research - 1 Sept 2015
Yang Juntang, Wang Rong, Liu Binyan, Xue Qizhen, Zhong Mengyu, Zeng Hao, Zhang Huidong
Abstract excerpt
Abasic sites (Apurinic/apyrimidinic (AP) sites), produced ∼ 50,000 times/cell/day, are very blocking and miscoding. To better understand miscoding mechanisms of abasic site for yeast DNA polymerase η, pre-steady-state nucleotide incorporation and LC-MS/MS sequence analysis of extension product were studied using pol η(core) (catalytic core, residues 1-513), which can completely eliminate the potential effects of...
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