Article
Inaccurate DNA synthesis in cell extracts of yeast producing active human DNA polymerase iota.
PloS one - 31 Jan 2011
Makarova Alena V, Grabow Corinn, Gening Leonid V, Tarantul Vyacheslav Z, Tahirov Tahir H, Bessho Tadayoshi, Pavlov Youri I
Abstract excerpt
Mammalian Pol ι has an unusual combination of properties: it is stimulated by Mn(2+) ions, can bypass some DNA lesions and misincorporates "G" opposite template "T" more frequently than incorporates the correct "A." We recently proposed a method of detection of Pol ι activity in animal cell extracts, based on primer extension opposite the template T with a high concentration of only two nucleotides, dGTP and dATP...
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