Article
A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing.
eLife - 10 Jul 2015
Liu Sunbin, Mozaffari-Jovin Sina, Wollenhaupt Jan, Santos Karine F, Theuser Matthias, Dunin-Horkawicz Stanislaw, Fabrizio Patrizia, Bujnicki Janusz M, Lührmann Reinhard, Wahl Markus C
Abstract excerpt
Prp3 is an essential U4/U6 di-snRNP-associated protein whose functions and molecular mechanisms in pre-mRNA splicing are presently poorly understood. We show by structural and biochemical analyses that Prp3 contains a bipartite U4/U6 di-snRNA-binding region comprising an expanded ferredoxin-like fold, which recognizes a 3'-overhang of U6 snRNA, and a preceding peptide, which binds U4/U6 stem II. Phylogenetic...
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