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Article

A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro.

FEBS letters - 4 Aug 2015

Miller Corwin, Bröcker Markus J, Prat Laure, Ip Kevan, Chirathivat Napon, Feiock Alexander, Veszprémi Miklós, Söll Dieter

Abstract excerpt

Incorporation of selenocysteine (Sec) in bacteria requires a UGA codon that is reassigned to Sec by the Sec-specific elongation factor SelB and a conserved mRNA motif (SECIS element). These requirements severely restrict the engineering of selenoproteins. Earlier, a synthetic tRNASec was reported that allowed canonical Sec incorporation by EF-Tu; however, serine misincorporation limited its scope. We report a...

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Source

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Identifiers

PMID: 26160755
DOI: 10.1016/j.febslet.2015.06.039
PMCID: PMC4782793

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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