Article
GFP Loss-of-Function Mutations in Arabidopsis thaliana.
G3 (Bethesda, Md.) - 6 Jul 2015
Fu Jason L, Kanno Tatsuo, Liang Shih-Chieh, Matzke Antonius J M, Matzke Marjori
Abstract excerpt
Green fluorescent protein (GFP) and related fluorescent proteins are widely used in biological research to monitor gene expression and protein localization in living cells. The GFP chromophore is generated spontaneously in the presence of oxygen by a multi-step reaction involving cyclization of the internal tripeptide Ser65 (or Thr65)-Tyr66-Gly67, which is embedded in the center of an 11-stranded β-barrel...
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