Development and applications of enhanced green fluorescent protein mutants.

doi:10.2144/98243rr01

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Article

Development and applications of enhanced green fluorescent protein mutants.

BioTechniques - 1 Mar 1998

Stauber R H, Horie K, Carney P, Hudson E A, Tarasova N I, Gaitanaris G A, Pavlakis G N

Abstract excerpt

The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upo...

Topics

3T3 Cells
Amino Acid Substitution
Animals
Biomarkers
Flow Cytometry
Gene Expression Regulation
Genes, Reporter
Green Fluorescent Proteins
Luminescent Proteins
Mice
Mutation
Recombinant Fusion Proteins
Spectrometry, Fluorescence
Transfection

Source

Open in PubMed

Identifiers

PMID: 9526659
DOI: 10.2144/98243rr01

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9526659-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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