Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Development and applications of enhanced green fluorescent protein mutants.

BioTechniques - 1 Mar 1998

Stauber R H, Horie K, Carney P, Hudson E A, Tarasova N I, Gaitanaris G A, Pavlakis G N

Abstract excerpt

The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to 3T3 Cells
Share to Biomarkers
Share to Flow Cytometry

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Expression Regulation

Share to Mutation

Share to Recombinant Fusion Proteins

Share to Transfection

Source

Identifiers

PMID: 9526659
DOI: 10.2144/98243rr01

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Development and applications of enhanced green fluorescent protein mutants.PMID 9526659Read ↗

Applications of the green fluorescent protein in cell biology and biotechnology.Semantic proximity Read ↗

Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells.Semantic proximity Read ↗

The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.Semantic proximity Read ↗

The utility of green fluorescent protein in transgenic plants.Semantic proximity Read ↗

Variants of green fluorescent protein GFPxm.Semantic proximity Read ↗

YGFP: a spectral variant of GFP.Semantic proximity Read ↗

Molecular biology and mutation of green fluorescent protein.Semantic proximity Read ↗

Molecular evolution of versatile derivatives from a GFP-like protein in the marine copepod Chiridius poppei.Semantic proximity Read ↗

[Chromoproteins of the green fluorescent protein family: properties and applications].Semantic proximity Read ↗

Improved version of the red fluorescent protein (drFP583/DsRed/RFP).Semantic proximity Read ↗

Synthesis and sequence optimization of GFP mutants containing aromatic non-natural amino acids at the Tyr66 position.Semantic proximity Read ↗

Comparative analysis of wildtype and mutant forms of GFP.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.