Article
Comparison of mutant forms of the green fluorescent protein as expression markers in Chinese hamster ovary (CHO) and Saccharomyces cerevisiae cells.
Journal of biotechnology - 11 Jun 1998
Natarajan A, Subramanian S, Srienc F
Abstract excerpt
Several green fluorescent protein (Gfp) mutants with increased cellular fluorescence compared to the wildtype protein have recently been generated. We have expressed and compared wildtype Gfp and mutants S65T, F100S/ M154T/V164A, F64L/S65T, and S65A/V68L/S72A under identical growth conditions in...
Topics
- Animals
- Biomarkers
- Bioreactors
- CHO Cells
- Cloning, Molecular
- Cricetinae
- Flow Cytometry
- Fluorescent Antibody Technique
- Gene Expression Regulation
- Genetic Markers
- Green Fluorescent Proteins
- Luminescent Proteins
- Microscopy, Confocal
- Mutation
- Promoter Regions, Genetic
- Saccharomyces cerevisiae
- Transfection