Article
Mitochondrial DNA mutations in single human blood cells.
Mutation research - 1 Sept 2015
Yao Yong-Gang, Kajigaya Sachiko, Young Neal S
Abstract excerpt
Determination mitochondrial DNA (mtDNA) sequences from extremely small amounts of DNA extracted from tissue of limited amounts and/or degraded samples is frequently employed in medical, forensic, and anthropologic studies. Polymerase chain reaction (PCR) amplification followed by DNA cloning is a routine method, especially to examine heteroplasmy of mtDNA mutations. In this review, we compare the mtDNA mutation...
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