Article
Rapid mapping of insertional mutations to probe cell wall regulation in Cryptococcus neoformans.
Fungal genetics and biology : FG & B - 1 Sept 2015
Esher Shannon K, Granek Joshua A, Alspaugh J Andrew
Abstract excerpt
Random insertional mutagenesis screens are important tools in microbial genetics studies. Investigators in fungal systems have used the plant pathogen Agrobacterium tumefaciens to create tagged, random mutations for genetic screens in their fungal species of interest through a unique process of trans-kingdom cellular transconjugation. However, identifying the locations of insertion has traditionally required...
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