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Article

Tn7-based genome-wide random insertional mutagenesis of Candida glabrata.

Genome research - 1 May 2003

Castano Irene, Kaur Rupinder, Pan Shihjung, Cregg Robert, Penas Alejandro De Las, Guo Nini, Biery Matthew C, Craig Nancy L, Cormack Brendan P

Abstract excerpt

We describe and characterize a method for insertional mutagenesis of the yeast pathogen Candida glabrata using the bacterial transposon Tn7. Tn7 was used to mutagenize a C. glabrata genomic fosmid library. Pools of random Tn7 insertions in individual fosmids were recovered by transformation into Escherichia coli. Subsequently, these were introduced by recombination into the C. glabrata genome. We found that C....

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Identifiers

PMID: 12695329
DOI: 10.1101/gr.848203
PMCID: PMC430877

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Source receipt: pubmed, retrieved 7 Sept 2026.

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