Article
Nonenriched PCR Versus Mutant-Enriched PCR in Detecting Selected Epidermal Growth Factor Receptor Gene Mutations Among Nonsmall-Cell Lung Cancer Patients.
Genetic testing and molecular biomarkers - 1 Aug 2015
Zaki Moyassar Ahmad, Ramadan Ragaa Abd El Kader, Mahmoud Mahmoud Ibrahim, El-Kaffash Dalal Mohammed, Assaad Rami Samir
Abstract excerpt
BACKGROUND: Biopsies obtained from lung cancers contain a mixture of cancerous and healthy tissues. The mutant-enriched polymerase chain reaction (ME-PCR) identifies low-level somatic DNA mutations within an excess wild-type sample. AIMS: This study aimed at comparing nonenriched PCR (NE-PCR) versus ME-PCR for the detection of two epidermal growth factor receptor (EGFR) gene mutations among nonsmall cell lung...
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