Article
High-speed droplet-allele-specific polymerase chain reaction for genotyping of single nucleotide polymorphisms.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Matsuda Kazuyuki, Honda Takayuki
Abstract excerpt
Single nucleotide alternations such as single nucleotide polymorphisms (SNPs) or single nucleotide mutations are useful genetic markers for molecular diagnosis, prognosis, drug response, and predisposition to diseases. Rapid identification of SNPs or mutations is clinically important, especially for determining drug responses and selection of molecular-targeted therapy. Here, we describe a rapid genotyping assay...
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