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Article

Ultra-precise detection of mutations by droplet-based amplification of circularized DNA.

BMC genomics - 9 Mar 2016

Wang Kaile, Ma Qin, Jiang Lan, Lai Shujuan, Lu Xuemei, Hou Yali, Wu Chung-I, Ruan Jue

Abstract excerpt

BACKGROUND: NGS (next generation sequencing) has been widely used in studies of biological processes, ranging from microbial evolution to cancer genomics. However, the error rate of NGS (0.1 % ~ 1 %) is still remaining a great challenge for comprehensively investigating the low frequency variations, and the current solution methods have suffered severe amplification bias or low efficiency. RESULTS: We creatively...

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Share to DNA, Bacterial
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Nucleic Acid Amplification Techniques

Share to Polymorphism, Single Nucleotide

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Identifiers

PMID: 26960407
DOI: 10.1186/s12864-016-2480-1
PMCID: PMC4784281

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