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Article

Droplet Digital PCR with EvaGreen Assay: Confirmational Analysis of Structural Variants.

Current protocols in human genetics - 1 Apr 2018

Tai Angela C, Parfenov Michael, Gorham Joshua M

Abstract excerpt

DNA structural variants can be analyzed by droplet digital PCR (ddPCR), a water-oil microfluidics and fluorescence technology to quantify target nucleic acids with extreme precision and sensitivity. Traditional ddPCR uses expensive fluorescent oligonucleotide probes that require extensive optimization. Here we describe a variation of ddPCR using a DNA-binding dye (EvaGreen), whose properties allow target products...

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Identifiers

PMID: 30040209
DOI: 10.1002/cphg.58
PMCID: PMC7001142

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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