Article
Novel high-speed droplet-allele specific-polymerase chain reaction: application in the rapid genotyping of single nucleotide polymorphisms.
Clinica chimica acta; international journal of clinical chemistry - 23 Sept 2013
Taira Chiaki, Matsuda Kazuyuki, Yamaguchi Akemi, Sueki Akane, Koeda Hiroshi, Takagi Fumio, Kobayashi Yukihiro, Sugano Mitsutoshi, Honda Takayuki
Abstract excerpt
BACKGROUND: Single nucleotide alterations such as single nucleotide polymorphisms (SNP) and single nucleotide mutations are associated with responses to drugs and predisposition to several diseases, and they contribute to the pathogenesis of malignancies. We developed a rapid genotyping assay based on the allele-specific polymerase chain reaction (AS-PCR) with our droplet-PCR machine (droplet-AS-PCR). METHODS:...
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