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Article

Rapid ABO genotyping by high-speed droplet allele-specific PCR using crude samples.

Journal of clinical laboratory analysis - 1 Jan 2018

Taira Chiaki, Matsuda Kazuyuki, Takeichi Naoya, Furukawa Satomi, Sugano Mitsutoshi, Uehara Takeshi, Okumura Nobuo, Honda Takayuki

Abstract excerpt

BACKGROUND: ABO genotyping has common tools for personal identification of forensic and transplantation field. We developed a new method based on a droplet allele-specific PCR (droplet-AS-PCR) that enabled rapid PCR amplification. We attempted rapid ABO genotyping using crude DNA isolated from dried blood and buccal cells. METHODS: We designed allele-specific primers for three SNPs (at nucleotides 261, 526, and...

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Source

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Identifiers

PMID: 28295603
DOI: 10.1002/jcla.22196
PMCID: PMC6816865

Genes

ABO

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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