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Article

Improving the Accuracy of Single-Nucleotide Variant Diagnosis Using On-Off Discriminating Primers.

Biosensors - 13 Mar 2023

Shin Juny, Jung Cheulhee

Abstract excerpt

Early detection of rare mutations through liquid biopsy can provide real-time information related to cancer diagnosis, prognosis, and treatment outcomes. Cell-free DNA samples used in liquid biopsies contain single-nucleotide variants (SNVs) with a variant allele frequency (VAF) of approximately ≤1%. Droplet digital polymerase chain reaction (ddPCR) is considered the gold standard of sequencing using liquid...

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PMID: 36979592
DOI: 10.3390/bios13030380
PMCID: PMC10046569

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