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Article

Multiplexed, targeted gene editing in Nicotiana benthamiana for glyco-engineering and monoclonal antibody production.

Plant biotechnology journal - 1 Feb 2016

Li Jin, Stoddard Thomas J, Demorest Zachary L, Lavoie Pierre-Olivier, Luo Song, Clasen Benjamin M, Cedrone Frederic, Ray Erin E, Coffman Andrew P, Daulhac Aurelie, Yabandith Ann, Retterath Adam J, Mathis Luc, Voytas Daniel F, D'Aoust Marc-André, Zhang Feng

Abstract excerpt

Biopharmaceutical glycoproteins produced in plants carry N-glycans with plant-specific residues core α(1,3)-fucose and β(1,2)-xylose, which can significantly impact the activity, stability and immunogenicity of biopharmaceuticals. In this study, we have employed sequence-specific transcription activator-like effector nucleases (TALENs) to knock out two α(1,3)-fucosyltransferase (FucT) and the two...

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Identifiers

PMID: 26011187
DOI: 10.1111/pbi.12403
PMCID: PMC11389102

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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